Response of Alfalfa with increasing degree of Potato Leafhopper Resistance to Applied Boron Fertilizer and Insecticide

Richard Leep, Tim Dietz and Peter Jeranyama

Department of Crop and Soil Sciences Michigan State University

Justification and Objectives

Producers, surviving in current markets, are faced with a number of limiting factors.  One of the greatest limiting factors is the cost and availability of farmland.  This limit requires the producer to maximize yield.  Many dairy and livestock producers in Michigan rely on alfalfa for up to 2/3 of their feed ration.  The cost of establishment of alfalfa is high because of input costs (i.e.seed, fertilizer and labor) and because of lost yields in the establishment year.  Producers need to optimize yield while without jeopardizing stand persistence.

 Boron is an important micronutrient that plays a role in root nodulation and protein synthesis in alfalfa.  There is delicate balance for optimum boron levels in the soil. Toxicity in alfalfa can occur when optimum levels are exceeded while yield and stand persistence may be affected if boron levels are low.  Soil moisture and pH conditions are negatively correlated with boron availability.  Leaching potential is greatest in course-textured soils, especially with high rainfall or irrigation.  Potato leafhopper damage and boron deficiency is exhibited as similar symptoms in the alfalfa plant.  This study will evaluate the response of alfalfa to boron application upon alfalfa yield, stand persistence, and forage quality. 

Procedure

Three varieties of alfalfa were seeded in a 3 factor randomized complete block design.  Boron, insecticide treatment and variety were the three factors in the trial.  Boron application rates were 0 or 2 lbs./acre.  Half of the plots received an application of insecticide every two weeks.  Alfalfa varieties were either not resistant to potato leafhopper (PLH), 25% PLH-R, or 50% PLH-R.  Soil samples were taken from three locations within each plot for boron analysis.  Harvest plant height, percent stand, yellowing score and growth stages were recorded at each cutting.  Samples were analyzed for forage quality.  Boron analysis of the plant tissue is currently underway.

Summary and Discussion

Dry conditions prevailed at the Kellogg Biological Station in the 1998-growing season.  In this, the establishment year, two cuttings were taken.  The first cutting was taken on July 7 and the second cutting on August 25.  Yield average for cut 1 was 1.24 tons of DM / acre and .62 tons of DM / acre yield average for cut 2 (Figure 1).  The application of an insecticide appeared to be the most critical treatment for increasing yield of alfalfa in this study (Figure 1).  There was no significant difference in yield between boron or variety treatments.  This may have been due in part to variability in soil texture resulting in yield differences because of subsequent soil moisture variability in a dry year.  Potato leafhopper populations peaked in late June and again in early August.  Yellowing or “hopper burn” was evident in the plots that were not treated with insecticide in both cut 1 and cut 2 (Figure 2).  There was an increase in crude protein for the varieties Vernal and Cleansweep when boron was applied compared to the other treatments.

In 1999 and 2000 growing seasons a four cutting system was utilized. For the two years an early first cutting date was 23 May and the latest fourth cutting was taken on 16 September. Dry matter yield data is presented in Figures 3-12.

In 1999, most treatments were not statistically different from each other except with insecticide and without boron. In the preceding treatment, Vernal was associated with higher yields compared with either Cleansweep or experimental line 54H69 (Figure 3). Cleansweep was associated with low yields in the no boron treatments. However, a boron application raised the yields of Cleansweep to be similar to those of experimental line 54H69 (Figure 4).  Cleansweep seems to respond to boron application better than other cultivars. In the no boron treatments Cleansweep yields were somewhat depressed compared with boron treatments in the third cutting (Figure 5). Total seasonal yields in 1999 seem to suggest that Cleansweep responded to boron application and that yields were reduced somewhat in the absence of boron. Data seem to suggest that insecticide application helped to maintain the yields of Vernal (Figure 7).

In 2000, Cleansweep was associated with higher yields when sprayed with insecticide and boron in the first through to third cutting (Figures 8-10). It is also worth noting that 54H69 was associated with a higher yield in the no boron and no insecticide treatments compared with the other two cultivars (Figure 8).  In the fourth cutting, Vernal produced a higher yield than the average of two remaining cultivars when sprayed with an insecticide in the absence of boron (Figure 11). Seasonal total yields were highest for Cleansweep with boron and insecticide (Figure 12). In the absence of boron and insecticide yields were highest for 54H69 compared with either of the two-remaining cultivars. When sprayed with insecticide and no boron yields were slightly higher with Vernal (Figure 12).   

Forage quality was evaluated only in 1998 and 1999 seasons after it had been shown that there were no discernable significant differences between boron treatments and among insecticide treatments.  In 1999, crude protein (CP) concentrations were not significantly different between boron treatments in Vernal and Cleansweep for both first and second cuttings (Table 2). However, 54H69 responded to boron treatment in the first cutting. A non-boron application was associated with lower CP concentration and these were lower by 0.6% (Table 2). For the same cultivar there were no significant differences in the second cutting.

Acid detergent fiber (ADF) concentrations were not significantly different among boron treatments for Vernal and 54H69 cultivars for the two cuttings under consideration (Table 2). Although the first cutting they were no significant differences between the two boron treatments (b vs. nb), in the second cutting boron was associated with a higher ADF concentration by factor 1.4% (Table 2). Acid detergent fiber concentrations (NDF) were similar among cultivars and between boron treatments. It is also worth noting that the second cutting was associated with lower NDF concentrations than the first cutting (Table 2).  Numerically speaking, NDF concentrations were slightly higher with boron than without boron.

Table 1

1998 KBS Boron Treatment Trial

Cut 1 - July 9

Cut 2 - Aug 25

C Protein

ADF

NDF

C Protein

ADF

NDF

Vernal

nb,ns

19.17

25.04

37.56

19.14

26.75

39.44

nb,s

19.51

24.5

36.42

18.38

27.14

40.55

b,ns

21.54

27.06

39.43

19.29

27.38

40.46

b,s

21.64

26.81

38.47

19.27

27.63

40.23

Cleansweep

nb,ns

20.35

24.36

36.14

20.03

24.75

37.10

nb,s

20.41

24.00

35.96

19.62

25.23

38.46

b,ns

22.87

24.65

35.1

20.60

24.96

37.76

b,s

22.72

24.75

35.93

20.93

24.52

37.44

54H69

nb,ns

22.99

24.42

34.41

20.39

25.89

38.49

nb,s

22.49

24.19

34.31

20.33

25.02

38.18

b,ns

21.96

23.66

34.59

19.97

26.01

38.62

b,s

22.12

24.56

35.07

20.44

26.23

38.73

CV%

8.14

8.82

4.24

4.73

5.96

4.61

LSD 5%

2.52

3.15

2.2

1.35

2.23

2.57

nb        no boron applied

b          boron applied (2 lbs./acre)

ns         no insecticide

s           insecticide applied

Table 2

1998 Established  KBS Boron Treatment Trial in 1999

Cut 1 – 26 May 1999

Cut 2 – 30 June 199

C Protein

ADF

NDF

C Protein

ADF

NDF

Vernal

nb,ns

21.32

32.62

44.59

23.11

29.41

38.62

nb,s

21.94

33.01

44.66

22.98

29.75

38.79

b,ns

20.86

33.60

45.08

22.45

29.71

39.34

b,s

21.31

34.44

45.64

22.49

30.34

39.84

LSDboron 5%

-

-

-

-

-

-

Cleansweep

nb,ns

21.90

31.62

43.15

23.24

26.13

35.57

nb,s

22.13

31.37

43.00

23.45

27.43

36.93

b,ns

21.25

32.24

43.91

22.94

28.59

37.65

b,s

22.21

31.48

42.94

24.36

27.81

36.82

LSDboron 5%

-

-

-

-

1.10

-

54H69

nb,ns

22.40

31.59

42.65

23.05

27.88

37.23

nb,s

21.96

31.84

43.52

23.75

27.33

36.48

b,ns

22.73

32.09

43.22